Physical and functional connection between auxilin and dynamin during endocytosis
نویسندگان
چکیده
منابع مشابه
Physical and functional connection between auxilin and dynamin during endocytosis.
During clathrin-mediated endocytosis, the GTPase dynamin promotes formation of clathrin-coated vesicles, but its mode of action is unresolved. We provide evidence that a switch in three functional states of dynamin (dimers, tetramers, rings/spirals) coordinates its GTPase cycle. Dimers exhibit negative cooperativity whereas tetramers exhibit positive cooperativity with respect to GTP. Our study...
متن کاملRegulating dynamin dynamics during endocytosis
Dynamin is a large GTPase that mediates plasma membrane fission during clathrin-mediated endocytosis. Dynamin assembles into polymers on the necks of budding membranes in cells and has been shown to undergo GTP-dependent conformational changes that lead to membrane fission in vitro. Recent efforts have shed new light on the mechanisms of dynamin-mediated fission, yet exactly how dynamin perform...
متن کاملA Feedback Loop between Dynamin and Actin Recruitment during Clathrin-Mediated Endocytosis
Clathrin-mediated endocytosis proceeds by a sequential series of reactions catalyzed by discrete sets of protein machinery. The final reaction in clathrin-mediated endocytosis is membrane scission, which is mediated by the large guanosine triophosphate hydrolase (GTPase) dynamin and which may involve the actin-dependent recruitment of N-terminal containing BIN/Amphiphysin/RVS domain containing ...
متن کاملRecruitment dynamics of GAK and auxilin to clathrin-coated pits during endocytosis.
Cyclin G-associated kinase (GAK), the ubiquitous form of the neuronal-specific protein auxilin 1, is an essential cofactor for Hsc70-dependent uncoating of clathrin-coated vesicles. Total internal reflectance microscopy was used to determine the timing of GAK binding relative to dynamin and clathrin binding during invagination of clathrin-coated pits. Following transient recruitment of dynamin ...
متن کاملDynamics of Dynamin during Clathrin Mediated Endocytosis in PC12 Cells
BACKGROUND Members of the dynamin super-family of GTPases are involved in disparate cellular pathways. Dynamin1 and dynamin2 have been implicated in clathrin-mediated endocytosis. While some models suggest that dynamin functions specifically at the point of vesicle fission, evidence also exists for a role prior to fission during vesicle formation and it is unknown if there is a role for dynamin...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 2006
ISSN: 0261-4189,1460-2075
DOI: 10.1038/sj.emboj.7601298